MTHFR C677T mutation affects adipogenic differentiation abilities of human bone marrow-derived mesenchymal stem cells

The effects of 5,10-Methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism on human bone marrow-derived mesenchymal stem cells (hBM-MSCs) viability, morphology, physiology and differentiation capacity were investigated in this study. For purpose, primary hBM-MSCs with wild type (WT, C/C), heterozygote (HTZ, C/T) homozygote (HMZ, T/T) for the MTHFR gene obtained ethical committee permission donor informed. Mutations detected using RFLP Sanger sequencing methods from genomic DNA isolated cells, colonization properties by CFU-F test proliferative differences MTT test. Adipogenic, osteogenic, chondrogenic induced to study changes their potentials, results statistically analyzed one-way ANOVA Graphpad Prism. A total 13 donors screened there no hBM-MSC markers vitro morphologies cells. While significant between WT HTZ as a result test, after 24 48 h. As tests, it was found that adipogenic significantly more HMZ than Osteogenic did not give results. these experiments, be affected genotype hBM-MSCs.